User manual LEICA CN

[. . . ] Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods ­ including any electronic systems and media ­ requires express prior permission in writing by Leica Microsystems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument. © Leica Microsystems Nussloch GmbH Published by: Leica Microsystems Nussloch GmbH Heidelberger Str. 17 - 19 D-69226 Nussloch Germany Telephone: +49 (0)6224 143-0 Fax: +49 (0)6224 143-200 Internet: http://www. histo-solutions. com Leica knife holder CE /CN 3 1. Operation Danger!For disinfection we recommend Leica Cryofect disinfection spray. Mounting the knife holder base 1. [. . . ] The knife edge should be at the same level as the back plate; even knives that have been greatly reduced in height by frequent resharpening (up to a height of 25 mm) can be properly height-adjusted and clamped in the knife holder CN. 15 16 16 Fig. 7 18 17 The anti-roll system is laterally adjustable (84-mm wide system only). A groove (17) in the axle (18) facilitates centering of the anti-roll system. Fig. 8 6 Instruction manual V 2. 2 ­ 08/2005 1. 19 Operation Finger guards The finger guards are integrated into the clamping jaws. The width of the finger guards is sufficient for knives up to 22 cm long. Once you finish sectioning, always cover the knife edge with the finger guards. Fig. 9 Prior to any work on knife and object, each time before changing the specimen block and during work breaks the knife edge must be covered with the finger guards! Shifting the clamping jaws 20 21 21 In the factory, the clamping jaws are mounted at a distance of 64 mm from each other. If necessary, the clamping jaws can be relocated to a distance of 84 mm. Fig. 10 23 22 To shift the clamping jaws the knife holder must be removed from the knife holder base. With a size 4 Allen key, loosen the screws (21) located at the bottom surface of the knife holder. Insert the longer of the two knife support bars (part of standard delivery). Never work with just one clamping jaw. Otherwise sufficient stability for the sectioning process cannot be guaranteed. In addition, long knives will no longer be sufficiently covered by the finger guard. Fig. 11 Leica knife holder CE /CN 7 1. Operation Knife support bar Ensure that the recess (24) is pointing away from the microtome. 12 Clearance angle adjustment The harder the specimen the wider the clearance angle. However: the wider the clearance angle the greater the compression of the sections. Also, remember that by adjusting the clearance angle, the position of the knife edge relative to the specimen may be altered. Therefore, always place the specimen block above the knife prior to adjusting the clearance angle. Otherwise, the specimen block may collide with the knife when it is moved back upwards. Clearance angle adjustment - knife holder CE 25 Fig. 13 Readjust the clearance angle with every new type of specimen material!The clearance angle scale is located on the left side of the knife holder. 11) on the right side of the knife holder and select a clearance angle setting of `0' (index marker (25) must be in line with figure `0'). If the sectioning results are not satisfactory, increase the clearance angle setting in 1° steps, until the desired result is achieved. 8 Instruction manual V 2. 2 ­ 08/2005 1. Clearance angle adjustment - knife holder CN Operation Prior to setting the clearance angle, the knife edge must be located exactly in the spin axis of the knife holder. 27 26 Fig. [. . . ] 18 10 Instruction manual V 2. 2 ­ 08/2005 1. Operation 33 Cleaning the knife holder CE Daily cleaning procedure 1. Tilt the anti-roll system (33) to the left, hold at lever (34) while doing so. Now you can remove the pressure plate (36) for cleaning (use alcohol or acetone). 34 36 35 For disinfection purposes, standard commercial detergents and disinfectants can be used ­ our recommendation is Leica Cryofect. Fig. 19 38 39 Cleaning the knife holder CN Daily cleaning procedure For daily cleaning, it is sufficient to remove section waste from the knife holder with a dry brush. [. . . ]